December 5th, 2017
•We present a protocol for double thymidine synchronization of HeLa cells followed by analysis using high resolution confocal microscopy. This method is key to obtaining large number of cells that proceed synchronously from S phase to mitosis, enabling studies on mitotic roles of multifunctional proteins which also possess interphase functions.
Related Videos
Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber Video (Video) | JoVE
Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination Video (Video) | JoVE
Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy Video (Video) | JoVE
Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae Video (Video) | JoVE
Synchronization of Caulobacter Crescentus for Investigation of the Bacterial Cell Cycle Video (Video) | JoVE
Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization Video (Video) | JoVE
A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis Video (Video) | JoVE
Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper Video (Video) | JoVE
Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions Video (Video) | JoVE
Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations Video (Video) | JoVE
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved