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Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

DOI :

10.3791/4239-v

September 17th, 2012

September 17th, 2012

10,340 Views

1Department of Hematology, Oncology and Stem Cell Transplantation, University Medical Center Freiburg

Metaphase to anaphase transition is triggered through anaphase-promoting complex (APC/C)-dependent ubiquitination and subsequent destruction of cyclin B. Here, we established a system which, following pulse-chase labeling, allows monitoring cyclin B proteolysis in entire cell populations and facilitates the detection of interference by the mitotic checkpoint.

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Proteolysis

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