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Live Cell Imaging of Chromosome Segregation During Mitosis

DOI :

10.3791/57389-v

March 14th, 2018

March 14th, 2018

9,011 Views

1Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration, 2Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research, US Food and Drug Administration

This protocol describes an easy and convenient method to label and visualize live chromosomes in mitotic cells using Histone2B-GFP BacMam 2.0 label and a spinning disc confocal microscopy system.

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Keywords Live Cell Imaging

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