In Vitro Enzyme Measurement to Test Pharmacological Chaperone Responsiveness in Fabry and Pompe Disease
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10:16 min
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December 20th, 2017
DOI :
December 20th, 2017
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Chapters in this video
0:05
Title
0:35
Site-directed Mutagenesis
1:45
Transformation and Screening for the Desired Clone
2:35
pcDNA3.1/GLA and pcDNA3.1/GAA Plasmid Transfection and Treatment of HEK293H
4:00
Cell Harvest
4:43
Homogenization by Freezing and Thawing and BCA Assay
6:17
Enzyme Activity Measurement with Artificial 4-Methylumbeliferyl Substrate
8:19
Results: Enzyme Activity Measurement Mutagenized 3 α-galactosidase and 3 Acid α-glucosidase
9:35
Conclusion
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