Perform ATP Hydrolysis Reaction with Purified Protein
3:09
Incubate Samples Containing Free Pi with Detection Reagent
5:44
Results: Quantitation of In Vitro ATPase Activity
6:59
Conclusion
Transcript
The overall goal of this procedure is to quantitate the in vitro ATPase activity of purified proteins for functional characterization. This method can be used to characterize new putative ATPases, evaluate the effective potential activators or inh
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We describe a basic protocol for quantitating in vitro ATPase activity. This protocol can be optimized based on the level of activity and requirements for a given purified ATPase.