January 7th, 2013
•The effects of activation of protein kinase C (PKC) isozymes on mitochondrial functions associated with respiration and oxidative phosphorylation and on cell viability are described. The approach adapts adenoviral technique to selectively overexpress PKC isozymes in primary cell culture and a variety of assays to determine mitochondrial functions and energy status of the cell.
Related Videos
Counting and Determining the Viability of Cultured Cells
Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer
Viability Assays for Cells in Culture
Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy
A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions
Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase (ERK) Signaling or Mitochondrial Events in Target Cells
Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved