JoVE Logo
Faculty Resource Center

Sign In

A Simple Fluorescence-based Reporter Assay to Identify Cellular Components Required for Ricin Toxin A Chain (RTA) Trafficking in Yeast

DOI :

10.3791/56588-v

December 15th, 2017

December 15th, 2017

7,857 Views

1Molecular and Cell Biology, Department of Biosciences and Center of Human and Molecular Biology (ZHMB), Saarland University

In the manuscript, we describe the use of a yeast-based fluorescence reporter assay to identify cellular components involved in the trafficking and killing processes of the cytotoxic A subunit of the plant toxin ricin (RTA).

Tags

Fluorescence based Reporter Assay

-- Views

Related Videos

article

Pyrosequencing: A Simple Method for Accurate Genotyping

article

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

article

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

article

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

article

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA

article

An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme

article

A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases

article

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay

article

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

article

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved