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Pyrosequencing: A Simple Method for Accurate Genotyping

27.2K Views

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13:06 min

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January 8th, 2008

DOI :

10.3791/630-v

January 8th, 2008

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1Department of Internal Medicine, Washington University in St. Louis

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Pyrosequencing
Genotyping
Pharmacogenetic Research
Human Genome Project
Data
Genotyping Methods
Polymorphisms
Tri allelic
Indels
Short repeat Polymorphisms
Allele Percentages
Methylation
Pooled Sample Assessment
Internal Quality Control
Single nucleotide Polymorphism Evaluation
Clinically Relevant Polymorphisms
Technique
Methodology

Chapters in this video

0:00

Title

0:40

Introduction

2:19

Preparing the PCR Product for Pyrosequencing

6:42

Pyrosequencing

6:45

Entering Assay Details Using Pyrosequencing 96A1.1 Software

8:36

Run Sample Plate

11:16

Examining Pyrosequencing Results

12:18

Conclusion

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