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Pyrosequencing: A Simple Method for Accurate Genotyping

DOI :

10.3791/630-v

13:06 min

January 8th, 2008

January 8th, 2008

26,831 Views

1Department of Internal Medicine, Washington University in St. Louis

Pyrosequencing(R) is one of the most thorough yet simple methods to date used to analyze polymorphisms. This method has led to rapid and efficient single-nucleotide polymorphism evaluation including many clinically relevant polymorphisms. The technique and methodology of Pyrosequencing is explained.

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Pyrosequencing

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