JoVE Logo
Faculty Resource Center

Sign In

A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue

DOI :

10.3791/51219-v

June 15th, 2014

June 15th, 2014

13,248 Views

1Regenerative Medicine Institute, Cedars-Sinai Medical Center

This protocol describes a novel mechanical chopping method that allows the expansion of spherical neural stem and progenitor cell aggregates without dissociation to a single cell suspension.  Maintaining cell/cell contact allows rapid and stable growth for over 40 passages.

Tags

CGMP

-- Views

Related Videos

article

Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells

article

Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue

article

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae

article

Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection

article

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

article

Progenitor-derived Oligodendrocyte Culture System from Human Fetal Brain

article

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

article

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

article

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

article

Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved