The overall goal of this experiment is to detect the real time engulfment and degradation kinetics of glial cells, such as astrocytes and microglia, through in vitro live-imaging of the phagocytosis of pH indicator-conjugated synaptosomes. This me
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This protocol presents an in vitro live-imaging phagocytosis assay to measure the phagocytic capacity of astrocytes. Purified rat astrocytes and microglia are used along with pH indicator-conjugated synaptosomes. This method can detect real-time engulfment and degradation kinetics and provides a suitable screening platform to identify factors modulating astrocyte phagocytosis.