Creating a Structurally Realistic Finite Element Geometric Model of a Cardiomyocyte to Study the Role of Cellular Architecture in Cardiomyocyte Systems Biology

DOI :

10.3791/56817-v

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April 18th, 2018

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9,577 Views

Vijay Rajagopal^1,2,3, Gregory Bass^2,3, Shouryadipta Ghosh^1,2,3, Hilary Hunt^2,4, Cameron Walker⁵, Eric Hanssen⁶, Edmund Crampin^2,3,4,7,8, Christian Soeller⁹

¹Cell Structure and Mechanobiology Group, University of Melbourne, ²Systems Biology Laboratory, Melbourne School of Engineering, University of Melbourne, ³Department of Biomedical Engineering, University of Melbourne, ⁴School of Mathematics and Statistics, Faculty of Science, University of Melbourne, ⁵Department of Engineering Science, University of Auckland, ⁶Advanced Microscopy Facility, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, ⁷ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, ⁸School of Medicine, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, ⁹Living Systems Institute, University of Exeter

Chapters

This protocol outlines a novel method to create a spatially detailed finite element model of the intracellular architecture of cardiomyocytes from electron microscopy and confocal microscopy images. The power of this spatially detailed model is demonstrated using case studies in calcium signaling and bioenergetics.

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