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Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1

DOI :

10.3791/54568-v

September 5th, 2016

September 5th, 2016

8,024 Views

1Inserm U1016, Institut Cochin, CNRS UMR 8104, Université Paris Descartes, Sorbonne Paris Cité

We describe a method to measure the velocity of phagosomes moving towards the cell center in living cells infected with or without the human immunodeficiency virus (HIV) type 1, using spinning disk confocal fluorescence microscopy to identify fluorescent infected cells and bright field microscopy to detect phagosomes.

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