April 5th, 2018
•Here we present two ultrafast protocols for reconstitution of membrane proteins into fusogenic proteoliposomes, and fusion of such proteoliposomes with target lipid bilayers for detergent-free delivery of these membrane proteins into the postfusion bilayer. The combination of these approaches enables fast and easily controlled assembly of complex multi-component membrane systems.
Tags
Related Videos
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro Video (Video) | JoVE
Detection of Detergent-sensitive Interactions Between Membrane Proteins Video (Video) | JoVE
In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers Video (Video) | JoVE
Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator Video (Video) | JoVE
Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins Video (Video) | JoVE
Detergent-assisted Reconstitution of Recombinant Drosophila Atlastin into Liposomes for Lipid-mixing Assays Video (Video) | JoVE
PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays Video (Video) | JoVE
Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time Video (Video) | JoVE
Transverse Sectioning of Mature Rice Oryza sativa L. Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support Video (Video) | JoVE
Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens Video (Video) | JoVE
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved