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Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies

DOI :

10.3791/50436-v

July 13th, 2013

July 13th, 2013

19,218 Views

1Department of Cell Biology, University of Texas Southwestern Medical Center at Dallas

Procedures for complete reconstitution of a prototype voltage-gated potassium channel into lipid membranes are described. The reconstituted channels are suitable for biochemical assays, electrical recordings, ligand screening and electron crystallographic studies. These methods may have general applications to the structural and functional studies of other membrane proteins.

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