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Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants

DOI :

10.3791/63177-v

December 21st, 2021

December 21st, 2021

1,964 Views

1Laboratory of Genetics, University of Wisconsin-Madison

Early development is dependent on maternally-inherited products, and the role of many of these products is currently unknown. Herein, we described a protocol that uses CRISPR-Cas9 to identify maternal-effect phenotypes in a single generation.

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