JoVE Logo
Faculty Resource Center

Sign In

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish Video (Video) | JoVE

DOI :

10.3791/64686-v

February 10th, 2023

February 10th, 2023

988 Views

1Department of Molecular Biosciences, The University of Texas at Austin, 2Department of Nutritional Sciences, The University of Texas at Austin

CRISPR-Cas technologies have revolutionized the field of genome editing. However, finding and isolating the desired germline edit remains a major bottleneck. Therefore, this protocol describes a robust method for quickly screening F0 CRISPR-injected zebrafish sperm for germline edits using standard PCR, restriction digest, and gel electrophoresis techniques.

-- Views

Related Videos

article

Novel RNA-Binding Proteins Isolation by the RaPID Methodology Video (Video) | JoVE

article

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii Video (Video) | JoVE

article

Detection of Copy Number Alterations Using Single Cell Sequencing Video (Video) | JoVE

article

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens Video (Video) | JoVE

article

Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells Video (Video) | JoVE

article

Mating-based Overexpression Library Screening in Yeast Video (Video) | JoVE

article

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells Video (Video) | JoVE

article

Screening Cotton Genotypes for Reniform Nematode Resistance Video (Video) | JoVE

article

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models Video (Video) | JoVE

article

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells Video (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved