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Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

DOI :

10.3791/56990-v

9:05 min

December 25th, 2017

December 25th, 2017

11,702 Views

1Department of Entomology and Riverside Center of Disease Vector Research, Institute for Integrative Genome Biology, University of California, Riverside, 2Section of Cell and Developmental Biology, Division of Biological Sciences, University of California, San Diego

Microinjection of Nasonia vitripennis embryos is an essential method for generating heritable genome modifications. Described here is a detailed procedure for microinjection and transplantation of Nasonia vitripennis embryos, which will greatly facilitate future genome manipulation in this organism.

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Embryo Microinjection

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