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Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

DOI :

10.3791/55219-v

February 25th, 2017

February 25th, 2017

16,051 Views

1Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, 2Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute

We describe a method to sort single mammalian cells and to quantify the expression of up to 96 target genes of interest in each cell. This method includes the use of internal qPCR standards to enable the estimation of absolute transcript counts.

Tags

Single cell Gene Expression Profiling

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