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CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus (AAV) and 2-Cell Embryo Electroporation

DOI :

10.3791/66069-v

March 15th, 2024

March 15th, 2024

686 Views

1Animal Modeling Core, University of Missouri, 2Comparative Medicine Program, University of Missouri, 3Rat Resource and Research Center, University of Missouri, 4Department of Veterinary Pathobiology, University of Missouri

This protocol presents an optimized approach for producing genetically modified rat models. Adeno-associated virus (AAV) is used to deliver a DNA repair template, and electroporation is used to deliver CRISPR-Cas9 reagents to complete the genome editing process in 2-cell embryo.

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