Preparation of Adherent Platelet and Vascular Cell Monolayers
3:56
Fluorescent Labeling and Perfusion of Leukocytes
6:32
Results: Use of Laminar Flow-based Assay to Investigate the Interactions of Leukocytes with Their Cellular Partners
8:09
Conclusion
Transcript
The overall goal of this experiment is to investigate the mechanisms behind the interactions of leukocytes to adherent vascular cells or platelets under flow conditions. This method can help answer key questions in the immunology field such as how
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Leukocytes avidly interact with vascular cells and platelets after vessel wall injury or during inflammation. Here, we describe a straightforward laminar flow-based assay to characterize the molecular mechanisms that underlie the interactions between leukocytes and their cellular partners.