Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers

10.4K Views

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08:44 min

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June 11th, 2018

DOI :

10.3791/57113-v

June 11th, 2018

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Laura Mercatali¹, Patrizia Serra², Giacomo Miserocchi¹, Chiara Spadazzi¹, Chiara Liverani¹, Alessandro De Vita¹, Giorgia Marisi³, Alberto Bongiovanni¹, Federica Recine¹, Akwilina Pangan⁴, Nestory Masalu⁴, Toni Ibrahim¹, Dino Amadori¹

¹Osteoncology and Rare Tumors Center, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy, ²Unit of Biostatistics and Clinical Trials, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy, ³Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy, ⁴Department of Oncology, Bugando Medical Center, Mwanza, Tanzania

Summary

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Keywords Dried Blood Spots

Serum Spots

Sample Storage

Cancer Biomarkers

Translational Research

Blood Sample Collection

DNA Extraction

Proteinase K

Lysis Buffer

Chapters in this video

0:04

Title

1:20

Blood Storage in Dried Blood Spots (DBS)

2:20

DNA Extraction Starting from DBS

4:51

Serum Storage for Dried Serum Spots (DSS)

6:02

ELISA Assay Starting from DSS

6:47

Results: Successful Storage of High Quality Blood and Serum at Room Temperature

7:38

Conclusion

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