JoVE Logo
Faculty Resource Center

Sign In

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

DOI :

10.3791/58194-v

January 7th, 2019

January 7th, 2019

8,019 Views

1Cancer Blood Disease Institute, Divisions of Experimental Hematology and Cancer Pathology, Cincinnati Children's Hospital Medical Center

Here, we describe protocols for the genetic and chemical validation of c-Fos and Dusp1 as a drug target in leukemia using in vitro and in vivo genetic and humanized mouse models. This method can be applied to any target for genetic validation and therapeutic development.

-- Views

Related Videos

article

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

article

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

article

Evaluating In Vitro DNA Damage Using Comet Assay

article

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

article

Evaluating the Role of Mitochondrial Function in Cancer-related Fatigue

article

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

article

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

article

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture

article

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

article

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved