Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

14.6K Views

•

11:48 min

•

April 24th, 2018

DOI :

10.3791/57133-v

April 24th, 2018

•

Yannig Gicquel*¹^,², Robin Schubert*³^,⁴^,⁵, Svetlana Kapis³, Gleb Bourenkov⁶, Thomas Schneider⁶, Markus Perbandt³^,⁴, Christian Betzel³^,⁴^,⁵, Henry N. Chapman¹^,²^,⁴, Michael Heymann¹^,⁷

¹Center for Free Electron Laser Science, DESY, ²Department of Physics, University of Hamburg, ³Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, University of Hamburg, ⁴The Hamburg Center for Ultrafast Imaging, University of Hamburg, ⁵Integrated Biology Infrastructure Life-Science Facility at the European XFEL (XBI), ⁶European Molecular Biology Laboratory, EMBL c/o DESY, ⁷Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry

Summary

Explore More Videos

Keywords Microfluidic Chips

X ray Diffraction

In Situ

Crystal

Protein Crystallization

Serial Crystallography

Dynamic Light Scattering

Chapters in this video

0:04

Title

0:38

Polydimethylsiloxane (PDMS) Mold Fabrication and In Situ X-ray Chip Fabrication

4:59

Fluid Delivery Access Ports and Surface Treatment

6:51

Protein Preparation and Crystallization

8:51

Results: Crystallization and In Situ X-ray Diffraction of Protein Crystals

10:41

Conclusion

Related Videos

07:54

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

9.3K Views

09:16

X-ray Powder Diffraction in Conservation Science: Towards Routine Crystal Structure Determination of Corrosion Products on Heritage Art Objects

15.8K Views

11:32

High Pressure Single Crystal Diffraction at PX^2

21.3K Views

09:16

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

14.1K Views

14:58

Quantifying X-Ray Fluorescence Data Using MAPS

10.6K Views

10:35

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

12.0K Views

07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

7.5K Views

09:36

In Situ Lithiated Reference Electrode: Four Electrode Design for In-operando Impedance Spectroscopy

8.7K Views

07:55

Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering

12.6K Views

07:26

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

9.0K Views