JoVE Logo
Faculty Resource Center

Sign In

Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes

DOI :

10.3791/57215-v

5:22 min

April 13th, 2018

April 13th, 2018

9,964 Views

1Koch Institute for Integrative Cancer Research at MIT, Department of Biology, Massachusetts Institute of Technology

Aneuploidy leads to genome instability, which eventually produces cell cycle-arrested cells with complex karyotypes. This paper provides a simple and convenient method to isolate aneuploid cells with complex karyotypes that cease to divide.

Tags

Cell Cycle arrested Cells

-- Views

Related Videos

article

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

article

Mesenchymal Stem Cell Isolation from Pulp Tissue and Co-Culture with Cancer Cells to Study Their Interactions

article

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

article

Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment

article

Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity

article

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

article

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples

article

Decellularization of the Murine Cardiopulmonary Complex

article

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

article

Generation of an Orthotopic Xenograft of Pancreatic Cancer Cells by Ultrasound-Guided Injection

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved