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Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format

DOI :

10.3791/57305-v

8:35 min

April 20th, 2018

April 20th, 2018

6,574 Views

1Department of Neurosurgery, NorthShore University HealthSystem, 2Midwestern University/Chicago College of Osteopathic Medicine, 3Independent Contractor

Here we present a method for a human in vitro model of stretch injury in a 96-well format on a timescale relevant to impact trauma. This includes methods for fabricating stretchable plates, quantifying the mechanical insult, culturing and injuring cells, imaging, and high content analysis to quantify injury.

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High speed Stretch Injury

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