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Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells

DOI :

10.3791/53197-v

October 10th, 2015

October 10th, 2015

11,167 Views

1Human Genetics and Cognitive Functions, Institut Pasteur, 2CNRS URA 2182 'Genes, synapses and cognition', Institut Pasteur, 3Human Genetics and Cognitive Functions, Université Paris Diderot, Sorbonne Paris Cité, 4Plateforme d' Imagerie Dynamique, Imagopole, CiTech, Institut Pasteur, 5Neuroplasticity and Therapeutics, CECS, I-STEM, AFM, Evry

Dendritic spines of pyramidal neurons are the sites of most excitatory synapses in mammalian brain cortex. This method describes a 3D quantitative analysis of spine morphologies in human cortical pyramidal glutamatergic neurons derived from induced pluripotent stem cells.

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Three dimensional Quantification

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