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Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research

DOI :

10.3791/64586-v

8:20 min

May 12th, 2023

May 12th, 2023

2,248 Views

1Department of Applied Stem Cell Technologies, TechMed Centre Enschede, University of Twente, 2Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, 3Department of Genetics, Harvard Medical School

The present protocol provides a step-by-step procedure for the reproducible generation, maintenance, and aging of cerebral organoids derived from human-induced pluripotent stem cells (iPSCs). This method enables culturing and maturing cerebral organoids for extended periods, which facilitates the modeling of processes involved in brain aging and age-related pathogenesis.

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Tissue Fabrication

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