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Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

DOI :

10.3791/57322-v

May 6th, 2018

May 6th, 2018

12,005 Views

1Mucosal Immunology and Biology Research Center, Division of Pediatric Gastroenterology and Nutrition, Massachusetts General Hospital, 2Department of Pediatrics, Harvard Medical School

This protocol enables the reader to analyze bile salt-induced biofilm formation in enteric pathogens using a multifaceted approach to capture the dynamic nature of bacterial biofilms by assessing adherence, extracellular polymeric substance matrix formation, and dispersion.

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Keywords Bile Salts

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