Cannula Implantation into the Cisterna Magna of Rodents

43.7K Views

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10:13 min

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May 23rd, 2018

DOI :

10.3791/57378-v

May 23rd, 2018

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Anna L.R. Xavier¹, Natalie Linea Hauglund¹, Stephanie von Holstein-Rathlou¹, Qianliang Li¹, Simon Sanggaard¹^,², Nanhong Lou³, Iben Lundgaard³^,⁴, Maiken Nedergaard¹^,³

¹Center for Translational Neuromedicine, Division of Glial Therapeutics, University of Copenhagen, ²Department of Anesthesiology, Yale School of Medicine, ³Center for Translational Neuromedicine, Division of Glial Therapeutics, University of Rochester Medical Center, ⁴Department of Experimental Medical Science, Wallenberg Center for Molecular Medicine, Lund University

Summary

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Keywords Cannula Implantation

Neurological Diseases

Tracer Delivery

Signaling Molecules

Artificial Cerebrospinal Fluid

Chapters in this video

0:04

Title

1:17

Cannula Preparation

1:53

Insertion of the Cannula into the Cisterna Magna for Chronic Injections of CSF Tracers

5:52

Injection of CSF Tracers

6:43

Injecting CSF Tracers in Anesthetized Mice with Chronic Cisterna Magna Cannulation

7:20

Injecting CSF Tracers in Awake Mice with Chronic Cisterna Magna Cannulation

8:05

Results: Analysis of CSF Tracer Distribution

9:02

Conclusion

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