Opening of the Cisternal Membrane and Identification of Landmarks and Zero Point
4:11
Injection of the Target
4:45
Closure of the Surgical Field
5:10
Results: Stereotaxic Surgical Approach in Mice
6:14
Conclusion
Transcript
This protocol provides step-by-step guidelines for a reproducible route of access to regions of interest in the caudal brainstem and the upper cervical cord. This technique increases precision when delivering small injection volumes to restricted
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Stereotaxic surgery to target brain sites in mice commonly involves access through the skull bones and is guided by skull landmarks. Here we outline an alternative stereotaxic approach to target the caudal brainstem and upper cervical spinal cord via the cisterna magna that relies on direct visualization of brainstem landmarks.