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Whole-Mount Immunofluorescence Staining, Confocal Imaging and 3D Reconstruction of the Sinoatrial and Atrioventricular Node in the Mouse

DOI :

10.3791/62058-v

December 22nd, 2020

December 22nd, 2020

4,013 Views

1University Hospital Munich, Department of Medicine I, Ludwig Maximilians University (LMU) Munich, 2Institute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich, Ludwig Maximilians University (LMU) Munich, 3German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance

We provide a step-by-step protocol for whole-mount immunofluorescence staining of the sinoatrial node (SAN) and atrioventricular node (AVN) in murine hearts.

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