Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum
16.4K Views
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09:22 min
•
April 13th, 2018
DOI :
April 13th, 2018
•Chapters in this video
0:04
Title
1:06
Preparation of Crude Sample (Either Plasma or Serum) by Pelleting Larger Vesicles and Cellular Debris
2:04
Digestion of Non-exosomal Blood Proteins and Removal of Small Proteins/Peptides by Centrifuge Ultrafiltration
4:21
Purification of Vesicles by Size Exclusion Chromatography (SEC)
5:45
Concentration of Purified Exosomes/Total Protein Quantification and Quantification/Sizing of Exosomes by Nanoparticle Tracking Analysis (NTA)
7:09
Results: Increased Vesicle Yield and Exosome-specific Markers After Purification
8:15
Conclusion
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