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Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

16.3K Views

09:22 min

April 13th, 2018

DOI :

10.3791/57467-v

April 13th, 2018


Transcript

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Protein Digestion

Chapters in this video

0:04

Title

1:06

Preparation of Crude Sample (Either Plasma or Serum) by Pelleting Larger Vesicles and Cellular Debris

2:04

Digestion of Non-exosomal Blood Proteins and Removal of Small Proteins/Peptides by Centrifuge Ultrafiltration

4:21

Purification of Vesicles by Size Exclusion Chromatography (SEC)

5:45

Concentration of Purified Exosomes/Total Protein Quantification and Quantification/Sizing of Exosomes by Nanoparticle Tracking Analysis (NTA)

7:09

Results: Increased Vesicle Yield and Exosome-specific Markers After Purification

8:15

Conclusion

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