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Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

DOI :

10.3791/52095-v

November 18th, 2014

November 18th, 2014

11,735 Views

1Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, 2Linda Crnic Institute for Down Syndrome, University of Colorado, Denver

MicroRNAs (miRNAs) are a widely conserved class of regulatory molecules. Here we describe a miRNA cloning method that relies upon two potent ligation steps followed by high-throughput sequencing. Our method permits accurate genome-wide quantitation of miRNAs.

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MiRNA Quantitation

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