JoVE Logo
Faculty Resource Center

Sign In

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

DOI :

10.3791/52095-v

November 18th, 2014

November 18th, 2014

11,722 Views

1Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, 2Linda Crnic Institute for Down Syndrome, University of Colorado, Denver

MicroRNAs (miRNAs) are a widely conserved class of regulatory molecules. Here we describe a miRNA cloning method that relies upon two potent ligation steps followed by high-throughput sequencing. Our method permits accurate genome-wide quantitation of miRNAs.

Tags

MiRNA Quantitation

-- Views

Related Videos

article

Gramicidin-based Fluorescence Assay; for Determining Small Molecules Potential for Modifying Lipid Bilayer Properties

article

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

article

Substrate Generation for Endonucleases of CRISPR/Cas Systems

article

RNA Secondary Structure Prediction Using High-throughput SHAPE

article

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

article

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

article

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

article

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

article

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

article

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved