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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

DOI :

10.3791/59901-v

August 21st, 2019

August 21st, 2019

7,051 Views

1Division of Medical Genetics, University of Washington School of Medicine

Here, we describe a step-by-step strategy for isolating small RNAs, enriching for microRNAs, and preparing samples for high-throughput sequencing. We then describe how to process sequence reads and align them to microRNAs, using open source tools.

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