JoVE Logo
Faculty Resource Center

Sign In

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

DOI :

10.3791/59901-v

August 21st, 2019

August 21st, 2019

7,072 Views

1Division of Medical Genetics, University of Washington School of Medicine

Here, we describe a step-by-step strategy for isolating small RNAs, enriching for microRNAs, and preparing samples for high-throughput sequencing. We then describe how to process sequence reads and align them to microRNAs, using open source tools.

Tags

Isolating

-- Views

Related Videos

article

Detection of Copy Number Alterations Using Single Cell Sequencing

article

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

article

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

article

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

article

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

article

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

article

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

article

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

article

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

article

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved