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Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting

6.5K Views

11:37 min

June 18th, 2018

DOI :

10.3791/57474-v

June 18th, 2018


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MicroRNA Transfer

Chapters in this video

0:04

Title

0:53

CD133 SC Isolation

6:00

Characterization of Freshly Isolated CD133 SCs

7:21

Preparation of Transfection Complexes

8:26

CD133 SCs Transfection and Analysis

10:03

Results: Successful Engineering of miR-loaded Magnetized CD133 Cells

11:02

Conclusion

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