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Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain

DOI :

10.3791/57511-v

May 7th, 2018

May 7th, 2018

11,686 Views

1Department of Physiology, David Geffen School of Medicine, University of California Los Angeles, 2Department of Neurology, David Geffen School of Medicine, University of California Los Angeles, 3Department of Neurobiology, David Geffen School of Medicine, University of California Los Angeles

Potassium ions contribute to the resting membrane potential of cells and extracellular K+ concentration is a crucial regulator of cellular excitability. We describe how to make, calibrate and use monopolar K+-selective microelectrodes. Using such electrodes enables the measurement of electrically evoked K+ concentration dynamics in adult hippocampal slices.

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Potassium Ion Selective Microelectrodes

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