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Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques

DOI :

10.3791/3136-v

September 27th, 2011

September 27th, 2011

15,109 Views

1Department of Pharmacology, University of Tennessee College of Medicine

Neurons are first characterized electrophysiologically. Then the cytoplasm from the recorded neuron is aspirated and subjected to reverse transcription-PCR analysis to detect the expression of mRNAs for neurotransmitter synthesis enzymes, ion channels, and receptors.

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Profiling

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