CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
2.2K Views
•
10:40 min
•
April 25th, 2022
DOI :
April 25th, 2022
•Chapters in this video
0:04
Introduction
0:58
Preparation of CRISPR Gene Editing and Guide RNA Design to Generate miRNA Cluster Knockout Cell Lines
2:06
Generation of Stable Lentiviral Cell Lines Carrying the DOX-Inducible Cas9 Expression Cassette
3:20
Performing CRISPR Reactions by Cas9 Induction and Synthetic Guide RNA Transfection of Cells Using a High-Throughput Format
7:22
PCR Genotyping of CRISPR Cell Lines Using Crude Cell Lysates
8:37
Results: High-Throughput CRISPR Experimental Design to Generate Multiple miR-888 Cluster Deletion Combinations
9:51
Conclusion
Related Videos
Microarray Analysis for Saccharomyces cerevisiae
13.5K Views
Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
12.3K Views
Analysis of Autophagy in Penicillium chrysogenum by Using Starvation Pads in Combination With Fluorescence Microscopy
8.5K Views
Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish
9.5K Views
Pupal and Adult Injections for RNAi and CRISPR Gene Editing in Nasonia vitripennis
5.1K Views
TGF-β-mediated Endothelial to Mesenchymal Transition (EndMT) and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing
4.9K Views
In Vitro Analysis of E3 Ubiquitin Ligase Function
4.9K Views
CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications
3.3K Views
Embryo Injection Technique for Gene Editing in the Black-Legged Tick, Ixodes scapularis
2.3K Views
Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis
2.2K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved