September 3rd, 2021
•Here, we present an optimized protocol for imaging entire ovaries for quantitative and qualitative analyses using whole-mount immunostaining, multiphoton microscopy, and 3D visualization and analysis. This protocol accommodates high-throughput, reliable, and repeatable processing that is applicable for toxicology, clinical diagnostics, and genomic assays of ovarian function.
Tags
Related Videos
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart
Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts
Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques
Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries
Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary
Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved