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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

DOI :

10.3791/2552-v

7:27 min

August 3rd, 2011

August 3rd, 2011

20,153 Views

1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco , 2Center for Reproductive Sciences, University of California San Francisco , 3Department of Urology, University of California San Francisco , 4Department of Cell and Tissue Biology, University of California San Francisco , 5Fluidigm Corporation, Fluidigm Corporation , 6Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, 7UCSF - Helen Diller Family Comprehensive Cancer Center, University of California San Francisco

Here we describe an optimized multiplex reverse transcriptase quantitative PCR (qRT-PCR) protocol in combination with a microfluidic platform as a cost and time effective high-throughput screening tool for microRNA (miRNA) expression levels, especially when working with limited amounts of sample.

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High Throughput MicroRNA Profiling

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