Conjugating the 3-Maleimido-PROXYL Nitroxide Spin Label
1:38
Preparing the NMR Sample for Measuring Intramolecular PRE
2:11
Setting Up the NMR Spectrometer and Experiment Specific Parameters
3:51
Setting Up the 1HN-T2 Experiment
4:58
Making a Diamagnetic Sample by Reducing Spin-Label with Ascorbic Acid
5:52
Results: 1HN-T2 Rates for Residues 171 - 264 of the Intrinsically Disordered Domain of EWSR1
6:30
Conclusion
Transcript
The paramagnetic relaxation enhancement technique can be utilized to characterize and measure intermolecular distances and quantify transient and/or lowly populated biomolecular interactions. Here, we have applied this technique to capture interac
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A protocol for the application of paramagnetic relaxation enhancement NMR spectroscopy to detect weak and transient inter- and intra-molecular interactions in intrinsically disordered proteins is presented.