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Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

DOI :

10.3791/60690-v

January 17th, 2020

January 17th, 2020

6,970 Views

1Department of Chemistry and Biochemistry, James Madison University, 2Biophysics Graduate Program, Ohio State University

We present an assay for easy quantification of metals introduced to samples prepared using immobilized metal affinity chromatography. The method uses hydroxynaphthol blue as the colorimetric metal indicator and a UV-Vis spectrophotometer as the detector.

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Keywords Metal Leaching

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