Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
7.9K Views
•
11:53 min
•
July 3rd, 2018
DOI :
July 3rd, 2018
•Chapters in this video
0:04
Title
0:54
TRPV1 Mutagenesis
2:19
Generating the Recombinant Bacmid and Baculovirus for Protein Expression
5:14
eTRPV1 and Single-cysteine Mutant Purification
7:18
eTRPV1 Single-cysteine Mutant Site-directed Spin Labeling
8:12
eTRPV1 Spin-labeled Single Cysteine Mutant Reconstitution
9:30
Results: Characterization of the Minimal Cysteine-less TRPV1 Construct and Single-cysteine Mutants used for Spectroscopic Analysis
11:08
Conclusion
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