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Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

DOI :

10.3791/63583-v

13:30 min

February 18th, 2022

February 18th, 2022

3,219 Views

1Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 2Department of Physics, Technical University Munich, 3Department of Cellular Physiology, Biomedical Center (BMC), Ludwig-Maximilians-Universität München

This protocol describes an iSCAT-based image processing and single-particle tracking approach that enables the simultaneous investigation of the molecular mass and the diffusive behavior of macromolecules interacting with lipid membranes. Step-by-step instructions for sample preparation, mass-to-contrast conversion, movie acquisition, and post-processing are provided alongside directions to prevent potential pitfalls.

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Mass sensitive Particle Tracking

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