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Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells

DOI :

10.3791/53806-v

March 3rd, 2016

March 3rd, 2016

9,933 Views

1The Center for Stem Cell Biology, Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, 2Cancer Biology and Genetics Program, Gerstner Sloan-Kettering Graduate School, Sloan-Kettering Institute for Cancer Research, 3Thermo Fisher Scientific

This work describes an in vitro differentiation protocol to produce pigmented, mature melanocytes from human pluripotent stem cells via a neural crest and melanoblast intermediate stage using a feeder-free, 25 day protocol.

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Feeder free Derivation

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