Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes

7.1K Views

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06:59 min

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September 27th, 2018

DOI :

10.3791/58134-v

September 27th, 2018

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Alexander Goedel*¹^,², Dorota M. Zawada*¹, Fangfang Zhang¹, Zhifen Chen³, Alessandra Moretti¹^,², Daniel Sinnecker¹^,²

¹Medical Department I, University Hospital Klinikum rechts der Isar, Technical University of Munich, ²German Centre for Cardiovascular Research (DZHK), Munich Heart Alliance, ³Beth Israel Deaconess Medical Center, Harvard Medical School

Summary

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Keywords Human Induced Pluripotent Stem Cell derived Ventricular Cardiomyocytes

Optical Action Potential Recordings

Genetically Encoded Voltage Sensors

Lentiviral Transduction

Cardiomyocyte Maintenance Medium

Tyrode s Solution

Epifluorescence Microscope

Chapters in this video

0:04

Title

0:50

Induced Pluripotent Stem Cell (iPS Cell)-derived Cardiomyocyte Preparation and Optical Membrane Potential Recording

3:25

Analysis

4:55

Results: Representative Optical Action Potential Measurement Analyses

6:36

Conclusion

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