JoVE Logo

Sign In

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses

12.8K Views

11:17 min

August 30th, 2018

DOI :

10.3791/58148-v

August 30th, 2018


Explore More Videos

Antibiotics

Chapters in this video

0:04

Title

1:05

Preparation of the Calibrators, Quality Control Samples, and Internal Standards Mix

2:29

Buffer Preparation for Chromatography

3:47

Sample Cleanup and HPLC-MS/MS Analysis

6:28

Quality Assessment and Quantification

8:14

Results: Data Analysis and Quantification of Piperacillin

9:44

Conclusion

Related Videos

article

11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

22.4K Views

article

08:57

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice

10.9K Views

article

09:06

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

6.8K Views

article

11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

10.0K Views

article

10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

9.5K Views

article

09:24

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

5.5K Views

article

08:38

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

16.8K Views

article

09:58

Spatial Quantification of Drugs in Pulmonary Tuberculosis Lesions by Laser Capture Microdissection Liquid Chromatography Mass Spectrometry (LCM-LC/MS)

9.0K Views

article

08:54

Validated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples

7.6K Views

article

06:30

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography

5.9K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved