Preparation of the Calibrators, Quality Control Samples, and Internal Standards Mix
2:29
Buffer Preparation for Chromatography
3:47
Sample Cleanup and HPLC-MS/MS Analysis
6:28
Quality Assessment and Quantification
8:14
Results: Data Analysis and Quantification of Piperacillin
9:44
Conclusion
Trascrizione
Therapeutic drug monitoring of antibiotics is increasingly recognized as an important element for the implementation of national and local antibiotic stewardship programs. To meet this increasing demand of antibiotic therapeutic drug monitoring in
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Here we present a tandem mass spectrometry-based protocol for the quantification of frequently used antibiotics in intensive care units, namely cefepime, meropenem, ciprofloxacin, moxifloxacin, linezolid, and piperacillin.