Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
7.2K Views
•
08:22 min
•
July 17th, 2018
DOI :
July 17th, 2018
•Explore More Videos
Chapters in this video
0:04
Title
0:50
FKBP12F36V-mVenus Purification
3:24
Preparation of Multiwell Plate Array
4:19
Calibration-free Confocal Acquisition
6:26
Results: FKBP12 Dimerizes upon Addition of BB Dimerizer Drug
7:23
Conclusion
Related Videos
09:18
Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
7.4K Views
08:43
A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
11.2K Views
08:29
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
6.1K Views
06:49
Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans
2.7K Views
07:22
The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)
3.1K Views
06:39
Analysis of Protein Complex Formation at Micromolar Concentrations by Coupling Microfluidics with Mass Photometry
1.9K Views
12:43
Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study
11.3K Views
10:43
Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis
6.7K Views
08:49
Photometric Protein Determination
133.1K Views
02:00
Protein Aggregate Formation Assay: A Method to Detect and Quantify Protein Aggregation in Cultured Cells upon Induction by Proteasome Inhibitor
216 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved