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Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans

DOI :

10.3791/63365-v

6:49 min

December 17th, 2021

December 17th, 2021

2,278 Views

1Bijvoet Centre for Biomolecular Research, Utrecht University

Here, a method is presented for the analysis of protein aggregation kinetics in the nematode Caenorhabditis elegans. Animals from an age-synchronized population are imaged at different time points, followed by semiautomated inclusion counting in CellProfiler and fitting to a mathematical model in AmyloFit.

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Protein Aggregation

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